Chromatography (BioRad)

Most chemical processes involve the separation of chemicals from a complex mixture.  Equally, recovering proteins inevitably requires some sort of purification step, whether extracting them from a waste stream, such as liquid dairy wastes or brewery residues, or from a fermentation specifically designed to produce target proteins.

At the BDC we have a wealth of knowledge and experience in the extraction and clean-up of proteins using a range of chromatographic techniques including ion exchange, affinity chromatography, hydrophobic interaction and gel filtration with three chromatography systems that allow us to work at different scales depending on the customer need:

AKTA Pure-25 protein purification system (0.001-25 mL per minute) is for fast purification of proteins, peptides, and nucleic acids from microgram to gram levels of target product.

BioRad Chromatography Skid is designed for the small-scale production/purification of pharmaceuticals or proteins. It has a column diameter of 100 mm and flow rates of between 5-120 L/hr.

Biotage Isolera LS system is a flash purification system designed for the separation of complex organic mixtures. It has cartridges instead of a column which are typically pre-packed. The flow rate of the Isolera system is 5-400 mL/min.

To learn more about how our chromatography systems can be put to use please contact our separation expert Raymond and Joe.

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