High performance liquid chromatography

High performance liquid chromatograph

High performance liquid chromatography

High Performance Liquid Chromatography (HPLC) is a flexible technique that allows separation and quantification of chemical substances found within a complex mixture.  For example it can be used for the quantification of sugars produced during biomass processing or for the determination of valuable chemical components in process streams or feedstocks.

HPLC involves injection of a liquid sample, containing the chemical(s) of interest, onto a chromatographic column. The sample is transported through the column by the flow of inert, liquid mobile phase. The column itself contains a solid stationary phase, through which the liquid flows. The chemicals being analysed interact with the stationary phase, with each chemical travelling through the column in a different time – this is known as the retention time.  The analytical value of HPLC is based on the comparison of retention times.

Technical specification: the gas chromatograph at the BDC is an HP1100 series, equipped with a 100-sample autosampler, quad-pump and both diode array (DAD) and evaporating light scattering (ELSD) detectors.   The DAD allows detection of many organic compounds, which can absorb ultraviolet or visible light. The ELSD is suitable for detection of compounds which do not absorb light (e.g. sugars and lipids).  Electronic data capture and analysis is performed using DataApex Clarity software.

To use the high performance liquid chromatograph or find out more about its capabilities, please contact us.

We have an extensive range of raw materials characterisation equipment, see below.

Raw materials characterisation

Agilent 2200 TapeStation nucleic acids system

Agilent 2200 TapeStation nucleic acids system

The 2200 TapeStation is ideal for the very rapid characterisation of DNA or RNA samples, including quality assessment, establishing DNA/RNA size ranges and quantification.

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Analytical equipment

Analytical equipment

We have a variety of analytical equipment available at the centre.

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Benchtop NMR analyser

Benchtop NMR analyser

The Benchtop NMR Analyser is based on Nuclear Magnetic Resonance (NMR) and allows for fast, accurate, reproducible and solvent-free measurement of oil, fat and moisture in oil seeds, seed cake and foodstuffs.

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Gas chromatography

Gas chromatography

Gas chromatography (GC) is a flexible technique that allows separation and quantification of chemical substances found within a complex mixture.

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High performance liquid chromatography

High performance liquid chromatography

High Performance Liquid Chromatography (HPLC) is a flexible technique that allows separation and quantification of chemical substances found within a complex mixture.

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Inductively coupled plasma mass spectrometer (ICP-MS)

Inductively coupled plasma mass spectrometer (ICP-MS)

The Agilent 7700x ICP-MS is capable of measuring metal and non-metal elements in concentrations of parts per trillion.

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Molecular analysis facilities

Molecular analysis facilities

We have established a set of molecular analysis labs to enable a variety of genetic analysis applications to be carried out.

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Microbiology facilities

Microbiology facilities

Our microbiology laboratory offers a range of equipment allowing users to safely handle, store and work with a variety of aerobic and anaerobic microbes, at ACDP hazard category II level.

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Oxidative stability analyser

Oxidative stability analyser

The Metrohm Rancimat helps us determine the oxidation stability of natural fats and oils. The instrument works on the principle of passing air through a test sample at an elevated temperature, causing accelerated aging of the sample.

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Water / liquid sample analysis

Water / liquid sample analysis

The Hache spectrophotometer offers a safe, easy and versatile system to measure various characteristics of liquid samples.

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